| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Truong, Elyssa (10)
|
NESSYS |
42.24 |
40.12 |
-2.12 |
| 2 |
Yun, Alana H (10)
|
NESSYS |
41.41 |
40.36 |
-1.05 |
| 3 |
Kelly, Elizabeth P (9)
|
NEMELY |
41.95 |
41.18 |
-0.77 |
| 4 |
Steinbergher, Cara A (10)
|
NESSYS |
42.01 |
41.30 |
-0.71 |
| 5 |
Farnsworth, Kaya M (10)
|
NEANA |
46.53 |
44.42 |
-2.11 |
| 6 |
Curley, Brynne M (9)
|
NEBYB |
46.46 |
44.44 |
-2.02 |
| 7 |
Breay, Naomi E (9)
|
NEMELY |
46.52 |
45.33 |
-1.19 |
| 8 |
Hazarika, Aneesa (9)
|
NEANA |
44.15 |
46.01 |
1.86 |
| 9 |
Rastelli, Sofia A (9)
|
NESSYS |
45.36 |
46.38 |
1.02 |
| 10 |
Duong, Athena (10)
|
NESSYS |
46.96 |
46.70 |
-0.26 |
| 11 |
Guan, Christina (9)
|
NESSYS |
47.06 |
48.77 |
1.71 |
| 12 |
Werner, Ariel M (9)
|
NESSYS |
51.91 |
48.94 |
-2.97 |
| 13 |
Russo, Lucinda P (9)
|
NESSYS |
48.56 |
49.00 |
0.44 |
| 14 |
Garcia, Olivia J (9)
|
NEANA |
50.29 |
49.04 |
-1.25 |
| 15 |
Sharma, Kanika (9)
|
NEANA |
|
52.30 |
- |
| 16 |
Alegria, Victoria I (10)
|
NEANA |
52.10 |
52.96 |
0.86 |
| 17 |
Serapiglia, Kaiden B (10)
|
NEANA |
|
53.50 |
- |
| 18 |
Dolinski, Madeline N (10)
|
NESSYS |
58.56 |
53.52 |
-5.04 |
| 19 |
Wright, Sienna C (9)
|
NEMELY |
54.68 |
54.68 |
0.00 |
|
Nussbaum, Bridget A (10)
|
NESSYS |
39.09 |
NS |
- |
|
Rigaud, Sophie L (10)
|
NESSYS |
46.22 |
NS |
- |